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human par2 applying mirus transit 293  (Mirus Bio)


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    Structured Review

    Mirus Bio human par2 applying mirus transit 293
    Human Par2 Applying Mirus Transit 293, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 97/100, based on 2904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+par2+applying+mirus+transit+293/TransIT+-293/pm32551018__ml0c00154_si_001-313-7-10
    Average 97 stars, based on 2904 article reviews
    human par2 applying mirus transit 293 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Transfection:

    Article Title: Discovery of Novel Non-Peptidic PAR2 Ligands
    Article Snippet: Measurement of PAR2- respectively Gq-stimulated IP1 formation was performed using the IP-One HTRF assay (CisBio, Codolet, France) according to the manufacturer’s protocol. .. In brief, HEK-293T cells transiently transfected with human PAR2 applying Mirus TransIT 293 as transfection reagent, were detached from the culture dish with Versene (Life Technologies, Darmstadt, Germany), seeded into black, clear flat bottom 384-well plates (Greiner, Frickenhausen, Germany) in assay medium at a density of 10,000 cells/well and maintained at 37 °C, 5% CO2 for 24 h. The medium was exchanged with stimulation buffer and cells were incubated with dilutions of the test compounds in stimulation buffer for 2 h at 37 °C, 5% CO2. ..

    Incubation:

    Article Title: Discovery of Novel Non-Peptidic PAR2 Ligands
    Article Snippet: Measurement of PAR2- respectively Gq-stimulated IP1 formation was performed using the IP-One HTRF assay (CisBio, Codolet, France) according to the manufacturer’s protocol. .. In brief, HEK-293T cells transiently transfected with human PAR2 applying Mirus TransIT 293 as transfection reagent, were detached from the culture dish with Versene (Life Technologies, Darmstadt, Germany), seeded into black, clear flat bottom 384-well plates (Greiner, Frickenhausen, Germany) in assay medium at a density of 10,000 cells/well and maintained at 37 °C, 5% CO2 for 24 h. The medium was exchanged with stimulation buffer and cells were incubated with dilutions of the test compounds in stimulation buffer for 2 h at 37 °C, 5% CO2. ..



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    97
    Mirus Bio human par2 applying mirus transit 293
    Human Par2 Applying Mirus Transit 293, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+par2+applying+mirus+transit+293/TransIT+-293/pm32551018__ml0c00154_si_001-313-7-10
    Average 97 stars, based on 1 article reviews
    human par2 applying mirus transit 293 - by Bioz Stars, 2026-10
    97/100 stars
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